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Diversity and taxonomy of members of genus Geastrum in Central America
Zehnálek, Petr ; Koukol, Ondřej (advisor) ; Tomšovský, Michal (referee)
In temperate zone, especially Europe and North America, is the genus Geastrum very well inspected group of Gasteromycetes with long lasting and comprehensive history of research. Our knowledge of the diversity in other areas of distribution, especially in tropics, is more than limited. The aim of this diploma thesis is to contribute for cognition of diversity of this genus in Panama, Costa Rica and Colombia I have collected 86 samples belonging to genus Geastrum during three expeditions to Panama (Chiriqué province) in the years 2015, 2016, 2017. I have characterized those based on morphological and molecular data. I have also studied morphology of 40 borrowed herbarium specimens from Costa Rica, Colombia and Panama. I have successfully sequenced four locuses of DNA from my own samples (ITS, LSU, RPB1 and ATP6). This method had lover efficiency in herbarium specimens due to present contaminations, age of specimens or probably badly stored. Molecular phylogenetic analysis was carried out by Bayesian method and its results have in agreement with morphologic traits shown many lineages new for Panama and moreover at least four new species completely new to science. Variability in species complexes (e.g. G. lageniforme, G. saccatum, G. velutinum) is presented in more detail with emphasis to lineages...
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